Selected publications (since 2011)



Wang XH, Müller WEG (2016) Three-dimensional morphology and gene expression in animals. In: Encyclopedia of Molecular Cell Biology and Molecular Medicine. (Meyers RA, ed) WILEY-VCH Press, Weinheim, in press

Wang XH, Schröder HC, Müller WEG (2016) Polyphosphate as a metabolic fuel in Metazoa: A foundational breakthrough invention for biomedical applications. Biotechnol. J. 11:11-30

Müller WEG, Schröder HC, Tolba E, Neufurth M, Diehl-Seifert B, Wang XH. (2016) Mineralization of bone-related SaOS-2 cells under physiological hypoxic conditions. FEBS J FEBS Journal 283: 74–87

Tolba E., Müller W.E.G., El-Hady B.M.A., Neufurth M., Wurm F., Wang S, Schröder H.C., Wang X.H. (2016) High biocompatibility and improved osteogenic potential of amorphous calcium carbonate/vaterite. J. Mat. Chem B 4, 376-386.

Müller WEG, Tolba E, Schröder HC, Muñoz-Espí R, Diehl-Seifert B, Wang XH (2016) Amorphous polyphosphate-hydroxyapatite: a morphogenetically active substrate for bone-related SaOS-2 cells in vitro. Acta Biomat 31: 358–367

Wang Xiaohong, Jian Huang, Kui Wang, Meik Neufurth, Heinz C. Schröder, Shunfeng Wang, Werner E.G. Müller (2016) The morphogenetically active polymer, inorganic polyphosphate complexed with GdCl3, as an inducer of hydroxyapatite formation in vitro. Biochem Pharmacol; in press



 Li Qiang, Wang XH, Korzhev M, Schröder HC, Link T, Tahir MN, Diehl-Seifert B and Müller WEG (2015) Potential biological role of laccase from the sponge Suberites domuncula as an antibacterial defense component. Biochim Biophys – Gen Subj 1850: 118-128

 Müller WEG, Tolba E, Schröder HC, Wang S, Glaßer G, Muñoz-Espí R, Link T, Wang XH (2015) A new polyphosphate calcium material with morphogenetic activity. Materials Letters 148:163-166

 Müller WEG, Tolba E, Schröder HC, Diehl-Seifert B and Wang XH (2015) Retinol encapsulated into amorphous Ca2+ polyphosphate nanospheres acts synergistically in MC3T3-E1 cells. Eur J Pharm Biopharm 93: 214-223

 Wang XH, Schröder HC, Müller WEG (2015) Properties and applications of biosilica enzymatically synthesized by aquatic/marine sponges. In: Kim SK (ed.) Functional Marine Biomaterials. Woodhead Publishing; in press.

 Wang XH and Müller W.E.G. (2015) Molecular morphogenesis: gene expression patterns in animals. In: Meyers RA (Ed) Encyclopedia of Molecular Cell Biology and Molecular Medicine: Developmental Biology. Wiley-VCH Verlag: Weinheim; in press.

 Müller WEG, Tolba E, Schröder HC and Wang XH (2015) Polyphosphate: a morphogenetically active implant material serving as metabolic fuel for bone regeneration. Macromolec Biosci; DOI: 10.1002/mabi.201500100.

 Müller WEG, Neufurth M, Huang J, Wang K, Feng Q, Schröder HC, Diehl-Seifert B, Muñoz-Espí R, Wang XH (2015) Non-enzymatic transformation of amorphous CaCO3 into calcium phosphate mineral after exposure to sodium phosphate in vitro: Implications for in vivo hydroxyapatite bone formation. ChemBioChem 16:1323-1332.

 Müller WEG, Tolba E, Feng Q, Schröder HC, Markl JS, Kokkinopoulou M and Wang XH (2015) Amorphous Ca2+ polyphosphate nanoparticles regulate the ATP level in bone-like SaOS-2 cells. J Cell Sci 128: 2202-2207

 Neufurth M, Wang XH, Tolba E, Dorweiler B, Schröder HC, Link T, Diehl-Seifert B, Müller WEG (2015) Modular small diameter vascular grafts with bioactive functionalities. PLoS ONE; in press



 Wang XH, Schröder HC, Grebenjuk V, Diehl-Seifert B, Mailänder V, Steffen R, Schloßmacher U and Müller WEG (2014) The marine sponge-derived inorganic polymers, biosilica and polyphosphate, as morphogenetically active matrices/scaffolds for differentiation of human multipotent stromal cells: Potential application in 3D printing and distraction osteogenesis. Marine Drugs 12:1131-1147

 Müller WEG, Schlossmacher U, Schröder HC, Lieberwirth I, Glasser G, Korzhev M, Neufurth M and Wang XH (2014) Enzyme-accelerated and structure-guided crystallization of Ca-carbonate: role of the carbonic anhydrase in the homologous system. Acta Biomaterialia 10: 450–462

 Müller WEG, Neufurth M, Schlossmacher U, Schröder HC, Pisignano D, Wang XH (2014) The sponge silicatein-interacting protein silintaphin-2 blocks calcite formation of calcareous sponge spicules at the vaterite stage. RSC Advances 4: 2577–2585.

 Müller WEG, Li Q, Schröder HC, Hönig N, Yuan DX, Grebenjuk VA, Mussino F, Giovine M and Wang XH (2014) Carbonic anhydrase: A key regulatory and detoxifying enzyme for Karst plants. Planta 239:213–229.

 Schröder HC, Wang XH, Schloßmacher U, Wiens M and Müller WEG (2014) Biogenic inorganic polysilicates (biosilica): Formation and biomedical applications. In: Müller WEG, Wang XH and Schröder HC (eds.) Biomedical Inorganic Polymers, Progress in Molecular and Subcellular Biology vol. 54; pp 197-234.

 Müller WEG, Schröder HC, Shen ZJ, Feng QL and Wang XH (2014) Inorganic polymers: Morphogenic inorganic biopolymers for rapid prototyping chain. In: Müller WEG, Wang XH and Schröder HC (eds.) Biomedical Inorganic Polymers, Progress in Molecular and Subcellular Biology vol. 54; pp 235-259.

 Wang XH, Schröder HC, Schloßmacher U and Müller WEG (2014) Inorganic polyphosphates: Biologically active biopolymers for biomedical applications. In: Müller WEG, Wang XH and Schröder HC (eds.) Biomedical Inorganic Polymers, Progress in Molecular and Subcellular Biology vol. 54; pp 261-294.

 Wang XH, Schröder HC, Schlossmacher U, Neufurth M, Feng Q, Diehl-Seifert B and Müller WEG (2014) Modulation of the initial mineralization process of SaOS-2 cells by carbonic anhydrase activators and polyphosphate. Calcif Tissue Int 94:495–509

 Daletos G, de Voogd NJ, Müller WEG, Wray V, Lin WH, Feger D, Kubbutat M, Aly AH and Peter Proksch (2014) Cytotoxic and protein kinase inhibiting Nakijiquinones and Nakijiquinols from the sponge Dactylospongia metachromia. J Nat Prod 77: 218-226.

 Wang XH, Schröder HC, Feng Q, Diehl-Seifert B, Grebenjuk VA, Müller WEG (2014) Isoquercitrin and polyphosphate co-enhance mineralization of human osteoblast-like SaOS-2 cells via separate activation of two RUNX2 cofactors AFT6 and Ets1. Biochemical Pharmacol 89:413-421

 Wang XH, Schröder HC and Müller WEG (2014) Biocalcite, a multifunctional inorganic polymer: Building block for calcareous sponge spicules and bioseed for the synthesis of calcium phosphate-based bone. Beilstein J. Nanotechnol. 5: 610–621.

 Wang XH, Schröder HC and Müller WEG (2014) Enzyme-based biosilica and biocalcite: biomaterials for the future in regenerative medicine. Trends Biotechnol 32:441-447.

 Wang XH, Schröder HC and Müller WEG (2014) Enzymatically synthesized inorganic polymers as morphogenetically active bone scaffolds: application in regenerative medicine. Int Rev Cell Mol Biol 313: 27-77.

 Müller WEG, Tolba E, Schröder HC, Diehl-Seifert B, Link T and Wang XH (2014) Biosilica-loaded poly(Ɛ-caprolactone) nanofibers mats provide a morphogenetically active surface scaffold for the growth and mineralization of the osteoclast-related SaOS-2 cells. Biotechnol. J. 9:1312-1321

 Wang XH, Müller WEG (2013) Three-Dimensional Morphology and Gene Expression in Animals. In: Encyclopedia of Molecular Cell Biology and Molecular Medicine. (Meyers RA, ed) WILEY-VCH Press, Weinheim, in press.

 Neufurth M, Wang XH, Schröder HC, Feng QL, Diehl-Seifert B, Ziebart T, Steffen R, Wang SF and Müller WEG (2014) Engineering a morphogenetically active hydrogel for bioprinting of bioartificial tissue derived from human osteoblast-like SaOS-2 cells. Biomaterials 35:8810-8819.

 Müller WEG, Link T, Schröder HC, Korzhev M, Neufurth M, Brandt D and Wang XH (2014) Dissection of the structure-forming from the structure-guiding activity of silicatein: a biomimetic molecular approach to print optical fibers. J Mater Chem B 2:5368-5377.

 Wang SF, Wang XH, Draenert FG, Albert O, Schröder HC, Mailänder V, Mitov G and Müller WEG (2014) Bioactive and biodegradable silica biomaterial for bone regeneration. Bone 67: 292–304.

 Müller WEG, Link T, Li Q, Schröder HC, Batel R, Blažina M, Grebenjuk VA and Wang XH (2014) A novel TiO2-assisted magnetic nanoparticle separator for treatment and inactivation of bacterial contaminants in aquatic systems. RSC Advances 4: 48267-48275.

 Wang XH, Tolba E, Schröder HC, Neufurth M, Feng Q, Diehl-Seifert B, Müller WEG (2014) Effect of bioglass on growth and biomineralization of SaOS-2 cells in hydrogel after 3D cell bioprinting. PLoS ONE  9(11): e112497. doi:10.1371/journal.pone.0112497



 Link T, Wang XH, Schloßmacher U, Feng QL, Schröder HC and Müller WEG (2013) An approach to a biomimetic bone scaffold: Increased expression of BMP-2 and of osteoprotegerin in SaOS-2 cells grown onto silica-biologized 3D printed scaffolds. RSC Advances 3:11140–11147

 Wang XH, Schröder HC, Diehl-Seifert B, Kropf K, Schlossmacher U, Wiens M and Müller WEG (2013) Dual effect of inorganic polymeric phosphate/polyphosphate on osteoblasts and osteoclasts in vitro. J Tissue Engin Regen Med 7:767-776.

 Müller WEG, Mugnaioli E, Schröder HC, Schloßmacher U, Giovine M, Kolb U and Wang XH (2013) Hierarchical composition of the axial filament from spicules of the siliceous sponge Suberites domuncula: From biosilica-synthesizing nanofibrils to structure- and morphology-guiding triangular stems. Cell Tissue Res 351:49-58

 Wang XH, Schloßmacher U, Schröder HC, and Müller WEG (2013) Biologically-induced transition of bio-silica sol to mesoscopic gelatinous flocs: a biomimetic approach to a controlled fabrication of bio-silica structures. Soft Matter 9:654-664.

 Wang XH, Schröder HC, Schloßmacher U, Jiang L, Korzhev M and Müller WEG (2013) Biosilica aging: From enzyme-driven gelation via syneresis to chemical/biochemical hardening. Biochim. Biophys. Acta [General Subjects] 1830: 3437–3446.

 Müller WEG, Schröder HC, Muth S, Gietzen S, Korzhev M, Grebenjuk VA, Wiens M, Schloßmacher U and Wang XH (2013) The silicatein-propeptide acts as inhibitor/modulator of self-organization during spicule axial filament formation. FEBS J 280:1693-1708

 Müller WEG, Schröder HC, Burghard Z, Pisignano D and Wang XH (2013) Silicateins - A novel paradigm in bioinorganic chemistry: Enzymatic synthesis of inorganic polymeric silica. Chemistry Eur. J. 19:5790-5804.

 Schloßmacher U, Schröder HC, Wang XH, Feng Q, Diehl-Seifert B, Neumann S, Trautwein A, Müller WEG (2013) Alginate/silica composite hydrogel as a potential morphogenetically active scaffold for three-dimensional tissue engineering. RSC Advances 3:11185–11194.

 Müller WEG, Wang XH, Grebenjuk V, Diehl-Seifert B, Steffen R, Schloßmacher U, Trautwein A, Neumann S and Schröder HC (2013) Silica as a morphogenetically active inorganic polymer: effect on the BMP-2-dependent and RUNX2-independent pathway in osteoblast-like SaOS-2 cells. Biomaterials Sci. 1: 669-678.

 Müller WEG, Schröder HC, Feng QL, Schloßmacher U, Link T and Wang XH (2013) Development of a morphogenetically active scaffold for three-dimensional growth of bone cells: biosilica/alginate hydrogel for SaOS-2 cell cultivation. J Tissue Engin Regener Med; doi: 10.1002/term.1745.

 Wiens M, Niem T, Elkhooly TA, Steffen R, Neumann S, Schloßmacher U and Müller WEG (2013) Osteogenic potential of a biosilica-coated P(UDMA-co-MPS) copolymer. J. Mater. Chem. B 1: 3339-3343

 Müller WEG, Schröder HC, Markl JS, Grebenjuk VA, Korzhev M, Steffen R and Wang XH (2013) Cryptochrome in sponges: a key molecule linking photoreception with phototransduction. J. Histochem. Cytochem. 61:814-832.

 Müller WEG, Schröder HC, Schlossmacher U, Grebenjuk VA, Ushijima H and Wang XH (2013) Induction of carbonic anhydrase in SaOS-2 cells, exposed to bicarbonate and consequences for calcium phosphate crystal formation. Biomaterials 34:8671-8680.

 Müller WEG, Schröder HC, Schlossmacher U, Neufurth M, Geurtsen W, Korzhev M and Wang XH (2013) The enzyme carbonic anhydrase as an integral component of biogenic Ca-carbonate formation in sponge spicules. FEBS Open Bio 3: 357-362.



 Wang XH, Schröder HC, Wiens M, Ushijima H, Müller WEG (2012) Bio-silica and bio-polyphosphate: applications in biomedicine (bone formation). Current Opinion Biotechnol 23:570–578.

 Jiang CS, Müller WEG, Schröder HC and Guo YW (2012) Disulfide- and multisulfide-containing metabolites from marine sources. Chem. Rev. 112, 2179–2207

 Wang XH, Schloßmacher U, Wiens M, Batel R, Schröder HC, Müller WEG (2012) Silicateins, silicatein interactors, and cellular interplay in sponge skeletogenesis: Formation of glass fiber-like spicules. FEBS J 279: 1721–1736.

 Müller WEG, Wang XH, Grebenjuk VA, Korzhev M, Wiens M, Schloßmacher U and Schröder HC (2012) Common genetic denominators for Ca++-based skeleton in Metazoa: Role of osteoclast-stimulating factor and of carbonic anhydrase in a calcareous sponge. PLoS ONE; 7(4): e34617. doi:10.1371/journal.pone.0034617.

 Schröder HC, Wang XH, Manfrin A, Yu SH, Grebenjuk VA, Korzhev M, Wiens M, Schloßmacher U, Müller WEG (2012) Silicatein: acquisition of structure-guiding and structure-forming properties during maturation from the pro-silicatein to the silicatein form. J. Biol. Chem 287:22196-22205.

 Schröder HC, Wang XH, Wiens M, Diehl-Seifert B, Kropf K, Schloßmacher U, Müller WEG (2012) Silicate modulates the cross-talk between osteoblasts (SaOS-2) and osteoclasts (RAW 264.7 cells): inhibition of osteoclast growth and differentiation. J Cellular Biochem 113:3197-3206

 Wang XH, Schröder HC, Wang K, Kaandorp JA and Müller WEG (2012) Genetic, biological and structural hierarchies during sponge spicule formation: From soft sol-gels to solid 3D silica composite structures. Soft Matter 8:9501–9518

 Polini A, Pagliara S, Camposeo A, Cingolani R, Wang XH, Schröder HC, Müller WEG, Pisignano D (2012) Optical properties of in-vitro biomineralised silica. Scientific Reports (Nature group) 2: 607; DOI: 10.1038/srep00607

 Müller WEG, Wang XH, Grebenjuk VA, Korzhev M, Wiens M, Schloßmacher U, Schröder HC (2012) Nocturnin in the demosponge Suberites domuncula: a potential circadian clock protein controlling glycogenin synthesis in sponges. Biochem J 448: 233-242



Müller WEG, Wang XH, Diehl-Seifert B, Kropf K, Schloßmacher U, Lieberwirth I, Glasser G, Wiens M and Schröder HC (2011) Inorganic polymeric phosphate/polyphosphate as an inducer of alkaline phosphatase and a modulator of intracellular Ca2+ level in osteoblasts (SaOS-2 cells) in vitro. Acta Biomaterialia 7; 2661-2671.

 Natalio F, Corrales TP, Panthöfer M, Schollmeyer D, Müller WEG, Kappl M, Butt HJ, Tremel W (2013) Flexible minerals: Self-assembled calcite spicules with extreme bending strength. Science 339:1298-1302.

 Wang XH, Wiens M, Divekar M, Grebenjuk VA, Schröder HC, Batel R, Müller WEG (2011) Isolation and characterization of a Mn(II)-oxidizing bacillus strain from the demosponge Suberites domuncula. Marine Drugs 9: 1-28.

 Schlossmacher U, Wiens M, Schröder HC, Wang XH, Jochum KP and Müller WEG (2011) Silintaphin-1: Interaction with silicatein during structureguiding biosilica formation. FEBS J 278: 1145-1155.

 Wiens M, Schröder HC, Wang XH, Link T, Steindorf D and Müller WEG (2011) Isolation of the silicatein-α interactor Silintaphin-2 by a novel solid-phase pull-down assay. Biochemistry 50: 1981-1990.

 Müller WEG, Wang XH, Wiens M, Schloßmacher U, Jochum KP, Schröder HC (2011) Hardening of bio-silica in sponge spicules involves an aging process after its enzymatic polycondensation: Evidence for an aquaporin-mediated water absorption. Biochim. Biophys. Acta [General Subjects] 1810:713–726.

 Wang XH, Wiens M, Schröder HC, Schloßmacher U, Pisignano D, Jochum KP, and Müller WEG (2011) Evagination of cells controls bio-silica formation and maturation during spicule formation in sponges. PLoS ONE 6: e20523. doi:10.1371/journal.pone.0020523.

 Wang XH, Gan L, Wiens M, Schloßmacher U, Schröder HC, Müller WEG (2012) Distribution of Microfossils Within Polymetallic Nodules: Biogenic Clusters Within Manganese Layers. Marine Biotechnol 14:96–105.

 Müller WEG, Binder M, von Lintig J, Guo YW, Wang XH, Kaandorp JA, Wiens M, Schröder HC (2011) Interaction of the retinoic acid signaling pathway with spicule formation in the marine sponge Suberites domuncula through activation of bone morphogenetic protein-1. Biochim. Biophys. Acta - General Subjects 1810: 1178–1194.

 Wiens M, Schröder HC, Korzhev M, Wang XH, Batel R and Müller WEG (2011) Inducible ASABF-type antimicrobial peptide from the sponge Suberites domuncula: microbicidal and hemolytic activity in vitro and toxic effect on molluscs in vivo. Marine Drugs 9:1969-1994.

 Wang XH, . Schröder HC, Wiens M, Gan L, Tremel W and Müller WEG. (2012) From biosilica of sponges (Demospongiae and Hexactinellida) to fabricated biomedical materials. In: Fattorusso E, Gerwick W, Taglialatela-Scafati O (Eds.) Handbook of Marine Natural Products. Springer-Press, Berlin; pp. 1259-1284.

 Wang XH, Schröder HC, Brandt D, Wiens M, Lieberwirth I, Glasser G, Schloßmacher U, Wang SF, Müller WEG (2011) Sponge bio-silica formation involves syneresis following polycondensation in vivo. ChemBioChem 12, 2316-2324.