Publications

Selected publications (since 2011)

2017

Müller WEG, Tolba E, Ackermann M, Neufurth M, Wang S, Feng Q, Schröder HC, Wang XH (2017) Fabrication of amorphous strontium polyphosphate microparticles that induce mineralization of bone cells in vitro and in vivo. Acta Biomater 50: 89-101.

Müller WEG, Neufurth M, Ackermann M, Tolba E, Wang SF, Feng QL, Schröder HC, Wang XH (2017) Fabrication of a new physiological macroporous hybrid biomaterial/bioscaffold material based on polyphosphate and collagen by freeze-extraction. J Mater Chem B, in press.

Wang XH, Ackermann M, Neufurth M, Wang SF, Schröder HC, Müller WEG (2017) Morphogenetically-active barrier membrane for guided bone regeneration, based on amorphous polyphosphate. Mar Drugs 15: 5. doi:10.3390/md15050142.

Wang XH, Guo YW, Tolba E, Kokkinopoulou M, Wiens M, Schröder HC, Müller WEG (2017) Two-armed activation of bone mineral deposition by the flavones baicalin and baicalein, encapsulated in polyphosphate microparticles. Am J Chin Med 45: 533-555.

Schröder HC, Tolba E, Diehl-Seifert B, Wang XH, Müller WEG (2017) Electrospinning of bioactive wound-healing nets. In: Müller WEG, Schröder HC, Wang XH (eds) BlueGenics – From gene to bioactive product: Exploiting marine genomics for an innovative and sustainable European blue biotechnology industry. Springer-Press, Berlin; Prog Mol Subcell Biol 55: 187-219.

Wang XH, Neufurth M, Tolba E, Wang S, Schröder HC, Müller WEG (2017) Biocalcite and carbonic acid activators. In: Müller WEG, Schröder HC, Wang XH (eds) BlueGenics – From gene to bioactive product: Exploiting marine genomics for an innovative and sustainable European blue biotechnology industry. Springer-Press, Berlin; Prog Mol Subcell Biol 55: 187-219.

Müller WEG, Wang XH, Schröder HC (2017) New target sites for treatment of osteoporosis. In: Müller WEG, Schröder HC, Wang XH (eds) BlueGenics – From gene to bioactive product: Exploiting marine genomics for an innovative and sustainable European blue biotechnology industry. Springer-Press, Berlin; Prog Mol Subcell Biol 55: 187-219.

2016

Wang XH, Müller WEG (2016) Molecular morphogenesis: Gene expression patterns in animals. In: Reviews in Cell Biology and Molecular Medicine (Meyers RA, ed) WILEY-VCH Press, Weinheim, in press. Vol 2/no 1:1-41; DOI: 10.1002/3527600906.mcb.200500041.pub2.

Wang XH, Schröder HC and Müller WEG (2016) Polyphosphate as a metabolic fuel in Metazoa: A foundational breakthrough invention for biomedical applications. Biotechnol J 11: 11-30.

Müller WEG, Schröder HC, Tolba E, Neufurth M, Diehl-Seifert B and Wang XH (2016) Mineralization of bone-related SaOS-2 cells under physiological hypoxic conditions. FEBS J 283: 74–87.

Tolba E, Müller WEG, El-Hady BMA, Neufurth M, Wurm F, Wang S, Schröder HC and Wang XH (2016) High biocompatibility and improved osteogenic potential of amorphous calcium carbonate/vaterite. J Mat Chem B 4: 376-386.

Müller WEG, Tolba E, Schröder HC, Muñoz-Espí R, Diehl-Seifert B and Wang XH (2016) Amorphous polyphosphate-hydroxyapatite: a morphogenetically active substrate for bone-related SaOS-2 cells in vitro. Acta Biomater 31: 358–367.

Wang XH, Huang J, Wang K, Neufurth M, Schröder HC, Wang S and Müller WEG (2016) The morphogenetically active polymer, inorganic polyphosphate complexed with GdCl3, as an inducer of hydroxyapatite formation in vitro. Biochem Pharmacol 102: 97-106.

Wang XH, Wang S, He F, Tolba E, Schröder HC, Diehl-Seifert B and Müller WEG (2016) Polyphosphate as a bioactive and biodegradable implant material: induction of bone regeneration in rats. Adv Engin Mat 18: 1406-1417.

Müller WEG, Neufurth M, Wang S, Tolba E, Schröder HC and Wang XH (2016) Morphogenetically active scaffold for osteochondral repair (polyphosphate/alginate/N,O-carboxymethyl chitosan). Eur Cells Mater J [eCM] 31:174-190.

Wang XH, Ackermann M, Wang S, Tolba E, Neufurth M, Feng Q, Schröder HC and Müller WEG (2016) Amorphous polyphosphate/amorphous calcium carbonate implant material with enhanced bone healing efficacy in a critical-size-defect in rats. Biomed Mater 11: 035005.

Schröder, Heinz; Grebenjuk, Vlad; Wang, Xiaohong; Müller, Werner (2016) Hierarchical architecture of sponge spicules: biocatalytic and structure-directing activity of silicatein proteins as model for bioinspired applications. Bioinspir Biomim 11: 041002. doi: 10.1088/1748-3190/11/4/041002.

Müller WEG, Ackermann M, Tolba E, Neufurth M, Wang S, Schröder HC, Wang XH (2016) A bio-imitating approach to fabricate an artificial matrix for cartilage tissue engineering using magnesium-polyphosphate and hyaluronic acid. RSC Adv 6:88559-88570.

Müller WEG and Wang XH (2016) Evolution of the mineralized animal skeletons: Formation of bone hydroxyapatite via amorphous Ca-carbonate and Ca-phosphate. Eur Cells Mater 32 Suppl. 3: 1473.

Wang XH, Ackermann M, Tolba E, Neufurth M, Wurm F, Feng Q, Wang S, Schröder HC, Müller WEG (2016) Artificial cartilage bio-matrix formed of hyaluronic acid and Mg2+-polyphosphate. Eur Cell Mater 32:271-283.

Wang XH, Ackermann M, Wang SF, Tolba E, Neufurth N, Feng QL, Schröder HC, W.E.G. Müller (2016) Amorphous polyphosphate/amorphous calcium carbonate implant material with enhanced bone healing efficacy in a critical-size defect in rats. Biomed Mater 11: 035005. doi: 10.1088/1748-6041/11/3/035005.

2015

Müller WEG, Schröder HC, Feng QL, Schloßmacher U, Link T and Wang XH (2015) Development of a morphogenetically active scaffold for three-dimensional growth of bone cells: biosilica/alginate hydrogel for SaOS-2 cell cultivation. J Tissue Eng Regen Med 9: E39–E50.

Wang XH, Schröder HC, Müller WEG (2015) Properties and applications of biosilica enzymatically synthesized by aquatic/marine sponges. In: Springer Handbook of Marine Biotechnology; Editor - Prof. Se-Kwon Kim. Heidelberg: Springer pp. 1265-1277.

Wang XH and Müller WEG (2015) Involvement of aquaporin channels in water extrusion from biosilica during maturation of sponge siliceous spicules. Biol Bull 229: 24–37.

Müller WEG, Tolba E, Schröder HC, Wang S, Glaßer G, Muñoz-Espí R, Link T and Wang XH (2015) A new polyphosphate calcium material with morphogenetic activity. Mater Lett 148: 163-166.

Müller WEG, Tolba E, Schröder HC, Diehl-Seifert B and Wang XH (2015) Retinol encapsulated into amorphous Ca2+ polyphosphate nanospheres acts synergistically in MC3T3-E1 cells. Eur J Pharm Biopharm 93: 214-223.

Wang XH, Schröder HC, Müller WEG (2015) Properties and applications of biosilica enzymatically synthesized by aquatic/marine sponges. In: Kim SK (ed.) Functional Marine Biomaterials. Woodhead Publishing Series in Biomaterials. Elsevier: Amsterdam Pp. 33-50.

Müller WEG, Tolba E, Schröder HC and Wang XH (2015) Polyphosphate: a morphogenetically active implant material serving as metabolic fuel for bone regeneration. Macromolec Biosci 15: 1182-1197.

Müller WEG, Neufurth M, Huang J, Wang K, Feng Q, Schröder HC, Diehl-Seifert B, Muñoz-Espí R and Wang XH (2015) Non-enzymatic transformation of amorphous CaCO3 into calcium phosphate mineral after exposure to sodium phosphate in vitro: Implications for in vivo hydroxyapatite bone formation. ChemBioChem 16: 1323-1332.

Müller WEG, Tolba E, Feng Q, Schröder HC, Markl JS, Kokkinopoulou M and Wang XH (2015) Amorphous Ca2+ polyphosphate nanoparticles regulate the ATP level in bone-like SaOS-2 cells. J Cell Sci 128: 2202-2207.

Neufurth M, Wang XH, Tolba E, Dorweiler B, Schröder HC, Link T, Diehl-Seifert B, Müller WEG (2015) Modular small diameter vascular grafts with bioactive functionalities. PLoS ONE PLoS ONE 10(7): e0133632; DOI:10.1371/journal.pone.0133632.

Müller WEG, Tolba E, Dorweiler B, Schröder HC, Diehl-Seifert B, Wang XH (2015) Electrospun bioactive mats enriched with Ca-polyphosphate/retinol nanospheres as potential wound dressing. Biochem Biophys Rep 3: 150-160.

Müller WEG, Tolba E, Schröder HC, Wang S, Glasser G, Diehl-Seifert B, Wang XH (2015) Biologizing titanium alloy implant material with morphogenetically active polyphosphate. RSC Adv 5: 75465-75473.

2014

Müller WEG, Schlossmacher U, Schröder HC, Lieberwirth I, Glasser G, Korzhev M, Neufurth M and Wang XH (2014) Enzyme-accelerated and structure-guided crystallization of Ca-carbonate: role of the carbonic anhydrase in the homologous system. Acta Biomater 10: 450–462

Müller WEG, Albert O, Schröder HC and Wang XH (2014) Bio-inorganic nanomaterials for biomedical applications (Bio-silica and Polyphosphate). In: B. Bhushan, D. Luo, S. Schricker, W. Sigmund and S. Zauscher (eds) Handbook of Nanomaterials Properties. Berlin Heidelberg: Springer-Press; pp. 389-408

Müller WEG, Neufurth M, Schlossmacher U, Schröder HC, Pisignano D, Wang XH (2014) The sponge silicatein-interacting protein silintaphin-2 blocks calcite formation of calcareous sponge spicules at the vaterite stage. RSC Adv 4: 2577-2585.

Schröder HC, Wang XH, Schloßmacher U, Wiens M and Müller WEG (2014) Biogenic inorganic polysilicates (biosilica): Formation and biomedical applications. In: Müller WEG, Wang XH and Schröder HC (eds.) Biomedical Inorganic Polymers, Progress in Molecular and Subcellular Biology vol. 54; pp 197-234.

Müller WEG, Schröder HC, Shen ZJ, Feng QL and Wang XH (2014) Inorganic polymers: Morphogenic inorganic biopolymers for rapid prototyping chain. In: Müller WEG, Wang XH and Schröder HC (eds.) Biomedical Inorganic Polymers, Progress in Molecular and Subcellular Biology vol. 54; pp 235-259.

Wang XH, Schröder HC, Schloßmacher U and Müller WEG (2014) Inorganic polyphosphates: Biologically active biopolymers for biomedical applications. In: Müller WEG, Wang XH and Schröder HC (eds.) Biomedical Inorganic Polymers, Progress in Molecular and Subcellular Biology vol. 54; pp 261-294.

Wang XH, Schröder HC, Schlossmacher U, Neufurth M, Feng Q, Diehl-Seifert B and Müller WEG (2014) Modulation of the initial mineralization process of SaOS-2 cells by carbonic anhydrase activators and polyphosphate. Calcif Tissue Int 94:495-509.

ang XH, Schröder HC, Feng Q, Diehl-Seifert B, Grebenjuk VA, Müller WEG (2014) Isoquercitrin and polyphosphate co-enhance mineralization of human osteoblast-like SaOS-2 cells via separate activation of two RUNX2 cofactors AFT6 and Ets1. Biochemical Pharmacol 89:413-421.

Wang XH, Schröder HC and Müller WEG (2014) Biocalcite, a multifunctional inorganic polymer: Building block for calcareous sponge spicules and bioseed for the synthesis of calcium phosphate-based bone. Beilstein J. Nanotechnol. 5: 610-621.

Wang XH, Schröder HC and Müller WEG (2014) Enzyme-based biosilica and biocalcite: biomaterials for the future in regenerative medicine. Trends Biotechnol 32:441-447.

Wang XH, Schröder HC and Müller WEG (2014) Enzymatically synthesized inorganic polymers as morphogenetically active bone scaffolds: application in regenerative medicine. Int Rev Cell Mol Biol 313: 27-77.

Müller WEG, Tolba E, Schröder HC, Diehl-Seifert B, Link T and Wang XH (2014) Biosilica-loaded poly(Ɛ-caprolactone) nanofibers mats provide a morphogenetically active surface scaffold for the growth and mineralization of the osteoclast-related SaOS-2 cells. Biotechnol. J. 9: 1312-1321.

Müller WEG, Link T, Schröder HC, Korzhev M, Neufurth M, Brandt D and Wang XH (2014) Dissection of the structure-forming from the structure-guiding activity of silicatein: a biomimetic molecular approach to print optical fibers. J Mater Chem B 2: 5368-5377.

Wang SF, Wang XH, Draenert FG, Albert O, Schröder HC, Mailänder V, Mitov G and Müller WEG (2014) Bioactive and biodegradable silica biomaterial for bone regeneration. Bone 67: 292-304.

2013

Wang XH, Schröder HC, Diehl-Seifert B, Kropf K, Schlossmacher U, Wiens M and Müller WEG (2013) Dual effect of inorganic polymeric phosphate/polyphosphate on osteoblasts and osteoclasts in vitro. J Tissue Engin Regen Med 7:767-776.

Müller WEG, Mugnaioli E, Schröder HC, Schloßmacher U, Giovine M, Kolb U and Wang XH (2013) Hierarchical composition of the axial filament from spicules of the siliceous sponge Suberites domuncula: From biosilica-synthesizing nanofibrils to structure- and morphology-guiding triangular stems. Cell Tissue Res 351:49-58.

Wang XH, Schloßmacher U, Schröder HC, and Müller WEG (2013) Biologically-induced transition of bio-silica sol to mesoscopic gelatinous flocs: a biomimetic approach to a controlled fabrication of bio-silica structures. Soft Matter 9:654-664.

Wang XH, Schröder HC, Schloßmacher U, Jiang L, Korzhev M and Müller WEG (2013) Biosilica aging: From enzyme-driven gelation via syneresis to chemical/biochemical hardening. Biochim. Biophys. Acta - General Subjects 1830: 3437–3446.

Müller WEG, Schröder HC, Muth S, Gietzen S, Korzhev M, Grebenjuk VA, Wiens M, Schloßmacher U and Wang XH (2013) The silicatein-propeptide acts as inhibitor/modulator of self-organization during spicule axial filament formation. FEBS J 280:1693-1708

Müller WEG, Wang XH, Jochum KP and Schröder HC (2013) Self-healing, an intrinsic property of biomineralization processes. IUBMB Life 65:382-396.

Wang XH, Schröder HC, Feng QL, Draenert F and Müller WEG (2013) The deep-sea natural products, biogenic polyphosphate (Bio-PolyP) and biogenic silica (Bio-Silica), as biomimetic scaffolds for bone tissue engineering: fabrication of a morphogenetically-active polymer. Mar Drugs 11:718-746.

Müller WEG, Schröder HC, Burghard Z, Pisignano D and Wang XH (2013) Silicateins - A novel paradigm in bioinorganic chemistry: Enzymatic synthesis of inorganic polymeric silica. Chemistry Eur. J. 19:5790-5804.

Schloßmacher U, Schröder HC, Wang XH, Feng Q, Diehl-Seifert B, Neumann S, Trautwein A, Müller WEG (2013) Alginate/silica composite hydrogel as a potential morphogenetically active scaffold for three-dimensional tissue engineering. RSC Adv 3:11185-11194.

Müller WEG, Wang XH, Grebenjuk V, Diehl-Seifert B, Steffen R, Schloßmacher U, Trautwein A, Neumann S and Schröder HC (2013) Silica as a morphogenetically active inorganic polymer: effect on the BMP-2-dependent and RUNX2-independent pathway in osteoblast-like SaOS-2 cells. Biomat Sci 1: 669-678.

Müller WEG, Wang XH, Proksch P, Perry CC, Osinga R, Gardères J, Schröder HC (2013) Principles of biofouling protection in marine sponges: A model for the design of novel biomimetic and bio-inspired coatings in the marine environment? Mar Biotechnol 15:375-398

Müller WEG, Schröder HC, Markl JS, Grebenjuk VA, Korzhev M, Steffen R and Wang XH (2013) Cryptochrome in sponges: a key molecule linking photoreception with phototransduction. J. Histochem. Cytochem. 61:814-832.

Müller WEG, Schröder HC, Schlossmacher U, Grebenjuk VA, Ushijima H and Wang XH (2013) Induction of carbonic anhydrase in SaOS-2 cells, exposed to bicarbonate and consequences for calcium phosphate crystal formation. Biomaterials 34:8671-8680.

Müller WEG, Schröder HC, Schlossmacher U, Neufurth M, Geurtsen W, Korzhev M and Wang XH (2013) The enzyme carbonic anhydrase as an integral component of biogenic Ca-carbonate formation in sponge spicules. FEBS Open Bio 3: 357-362.

Wang XH, Schloßmacher U, Schröder HC and Müller WEG (2013) A milestone in biomineralization: From a structural assessment to a genetic concept of mineral formation. Or: How the enzyme silicatein forms a solid silica rod. In: Stephen J. Clarson [ed] ACS Symposium Series. Progress in Silicones and Silicone-Modified Materials; Vol. 1154, Chapter 11, pp 157-176.

2012

Wang XH, Schröder HC, Wiens M, Gan L, Tremel W and Müller WEG. (2012) From biosilica of sponges (Demospongiae and Hexactinellida) to fabricated biomedical materials. In: Fattorusso E, Gerwick W, Taglialatela-Scafati O (Eds.) Handbook of Marine Natural Products. Springer-Press, Berlin; pp. 1259-1284.

Wang XH, Wiens M, Schloßmacher U, Jochum KP, Schröder HC, Müller WEG (2012) Bio-sintering/bio-fusion of silica in sponge spicules: a review. Adv Biomat 14: B4-B12.

Wang XH, Schröder HC, Wiens M, Ushijima H, Müller WEG (2012) Bio-silica and bio-polyphosphate: applications in biomedicine (bone formation). Curr Opin Biotechnol 23:570–578.

Wang XH, Schröder HC, Wiens M, Schloßmacher U, and Müller WEG (2012) Biosilica: Molecular biology, biochemistry and function in demosponges as well as its applied aspects for tissue engineering. In: Advances In Marine Biology - Advances in Sponge Science: Physiology, Chemical and Microbial Diversity, Biotechnology. Volume 62; pp. 231-271.

Wang XH, Schloßmacher U, Wiens M, Batel R, Schröder HC, Müller WEG (2012) Silicateins, silicatein interactors, and cellular interplay in sponge skeletogenesis: Formation of glass fiber-like spicules. FEBS J 279: 1721-1736.

Müller WEG and Wang XH (2012) Silicatein: from chemical through enzymatic silica formation, to synthesis of biomimetic nanomaterials. FEBS J (Minireview Series) [A short overview of the series] 279: 1709.

Müller WEG, Wang XH, Grebenjuk VA, Korzhev M, Wiens M, Schloßmacher U and Schröder HC (2012) Common genetic denominators for Ca++-based skeleton in Metazoa: Role of osteoclast-stimulating factor and of carbonic anhydrase in a calcareous sponge. PLoS ONE; 7(4): e34617. doi:10.1371/journal.pone.0034617.

Schröder HC, Wang XH, Wiens M, Diehl-Seifert B, Kropf K, Schloßmacher U, Müller WEG (2012) Silicate modulates the cross-talk between osteoblasts (SaOS-2) and osteoclasts (RAW 264.7 cells): inhibition of osteoclast growth and differentiation. J Cellular Biochem 113:3197-3206.

Wang XH, Schröder HC, Wang K, Kaandorp JA and Müller WEG (2012) Genetic, biological and structural hierarchies during sponge spicule formation: From soft sol-gels to solid 3D silica composite structures. Soft Matter 8:9501-9518.

Polini A, Pagliara S, Camposeo A, Cingolani R, Wang XH, Schröder HC, Müller WEG, Pisignano D (2012) Optical properties of in-vitro biomineralised silica. Sci Rep (Nature group) 2: 607; DOI: 10.1038/srep00607.

Schröder HC, Wang XH, Manfrin A, Yu SH, Grebenjuk VA, Korzhev M, Wiens M, Schloßmacher U, Müller WEG (2012) Silicatein: acquisition of structure-guiding and structure-forming properties during maturation from the pro-silicatein to the silicatein form. J. Biol. Chem 287:22196-22205.

Müller WEG, Wang XH, Grebenjuk VA, Korzhev M, Wiens M, Schloßmacher U, Schröder HC (2012) Nocturnin in the demosponge Suberites domuncula: a potential circadian clock protein controlling glycogenin synthesis in sponges. Biochem J 448: 233-242.

Müller WEG, Wang XH, Guo YW, Schröder HC (2012) Potentiation of the cytotoxic activity of copper by polyphosphate on biofilm-producing bacteria: A bioinspired approach. Mar Drugs 10: 2369-2387.

Pagliara S, Polini A, Camposeo A, Schröder HC, Müller WEG, and Pisignano D (2012) Electrical properties of in vitro biomineralized recombinant silicatein deposited by microfluidics. Appl Phys Lett 101:193702.

2011

Müller WEG, Wang XH, Wiens M, Schloßmacher U, Jochum KP, Schröder HC (2011) Hardening of bio-silica in sponge spicules involves an aging process after its enzymatic polycondensation: Evidence for an aquaporin-mediated water absorption. Biochim Biophys Acta - General Subjects 1810:713–726.

Wang XH, Wiens M, Schröder HC, Schloßmacher U, Pisignano D, Jochum KP, and Müller WEG (2011) Evagination of cells controls bio-silica formation and maturation during spicule formation in sponges. PLoS ONE 6: e20523. doi:10.1371/journal.pone.0020523.

Müller WEG, Binder M, von Lintig J, Guo YW, Wang XH, Kaandorp JA, Wiens M, Schröder HC (2011) Interaction of the retinoic acid signaling pathway with spicule formation in the marine sponge Suberites domuncula through activation of bone morphogenetic protein-1. Biochim Biophys Acta - General Subjects 1810: 1178–1194.

Wang XH, Schröder HC, Brandt D, Wiens M, Lieberwirth I, Glasser G, Schloßmacher U, Wang SF, Müller WEG (2011) Sponge bio-silica formation involves syneresis following polycondensation in vivo. ChemBioChem 12: 2316-2324.

Polini A, Pagliara S, Camposeo A, Biasco A, Schröder HC, Müller WEG, Pisignano D (2011) Biosilica electrically-insulating layers by soft lithography-assisted biomineralisation with recombinant silicatein. Adv Mater 23: 4674–4678.

Müller WEG, Wang XH, Diehl-Seifert B, Kropf K, Schloßmacher U, Lieberwirth I, Glasser G, Wiens M and Schröder HC (2011) Inorganic polymeric phosphate/polyphosphate as an inducer of alkaline phosphatase and a modulator of intracellular Ca2+ level in osteoblasts (SaOS-2 cells) in vitro. Acta Biomaterialia 7; 2661-2671.

Wang XH, Wiens M, Divekar M, Grebenjuk VA, Schröder HC, Batel R, Müller WEG (2011) Isolation and characterization of a Mn(II)-oxidizing bacillus strain from the demosponge Suberites domuncula. Marine Drugs 9: 1-28.

Schlossmacher U, Wiens M, Schröder HC, Wang XH, Jochum KP and Müller WEG (2011) Silintaphin-1: Interaction with silicatein during structure-guiding biosilica formation. FEBS J 278: 1145-1155.